London Bridge, the railway station and nearby streets were sealed off on Tuesday afternoon while police investigated a suspicious vehicle.
The evacuation began at about 2.15pm and cordons were lifted soon after 3.35pm.
Police closed parts of London Bridge, Borough High Street, Duke Street Hill and Tooley Street while they investigated a suspicious vehicle.
Rail services were halted and buses were turned round at Elephant & Castle.
Southwark Police said that the incident was "found to be not suspicious".
Southeastern warned rail passengers to expect delays for the rest of the day.
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